To test the functionality of the siRNA machinery, E. Once RNAi-related genes were identified, nuclease activities in hemolymph were investigated through an ex vivo assay. To do this, total mRNA of all developmental stages was sequenced on an Illumina platform, followed by a de novo assembly, gene annotation and RNAi-related gene identification. Therefore, the aim of this study was to test whether the Neotropical brown stinkbug Euschistus heros has the main RNAi core genes and whether the supply of dsRNA could generate an efficient gene silencing response.
For the success of this technique, the presence of core RNAi genes and a functional silencing machinery is essential. Over the past few years, the use of RNA interference (RNAi) for insect pest management has attracted considerable interest in academia and industry as a pest-specific and environment-friendly strategy for pest control.
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